• Test Code:
  • Department:
  • Test Synonyms:
    ROS1 kinase markerROSROS 1
  • CPT Code(s):

Gene fusions involving ROS1 are present in 1-2% of non-small cell lung carcinomas, primarily adenocarcinomas. Several partner genes (FIG, SLC34A2, CD74) have been identified.  Resulting activation of ROS1 kinase activity appears to be a principal growth driver in these tumors. This kinase is sensitive to crizotinib, and patients with ROS1-fusion positive tumors have shown responses to this inhibitor. A break-apart FISH probe to the ROS1 locus can be used to screen for these fusions by FISH.

Recommended Use:
The ROS1 FISH test is recommended for screening NSCLC cases that are negative for EGFR mutation and ALK fusion.


FISH is performed on sections of formalin-fixed, paraffin-embedded tumor tissue.   Slides are prepared per standard protocols and 100 interphase cells are scored.

Specimen Requirements:

Formalin-fixed paraffin-embedded tumor block or FFPE slides:  

  • If sending slides, please include H&E stained slide cut from same block
  • Preferred slice thickness is 4-5 microns on positively charged slides. 
  • Please submit 3-5 slides.  Store at room temperature.
  • Contact Client Services at (855) 535-1522 for shipping supplies and instructions.

Test Performed (Days):


Turn Around Time:

5-7 days

Shipment Sensitivity Requirements:

  • Ship via overnight express, using the FedEx priority overnight label provided. 
  • Contact Client Services at (855) 535-1522 for shipping kits and instructions.


Additional Info:

The Knight Cancer Institute at Oregon Health & Science University is a pioneer in the field of precision cancer medicine. The institute's director, Brian Druker, M.D., helped prove it was possible to shut down just the cells that enable cancer to grow. This breakthrough has made once-fatal forms of the disease manageable and transformed how cancer is treated. The OHSU Knight Cancer Institute is the only National Cancer Institute-designated Cancer Center between Sacramento and Seattle – an honor earned only by the nation's top cancer centers. It is headquarters for one of the National Cancer Institute's largest research collaboratives, SWOG, in addition to offering the latest treatments and technologies as well as hundreds of research studies and clinical trials.

Learn More