• Test Code:
  • Department:
    Cancer FISH
  • Test Synonyms:
    ROS1 kinase markerROSROS 1
  • CPT Code(s):

Gene fusions involving ROS1 are present in 1-2% of non-small cell lung carcinomas, primarily adenocarcinomas. Several partner genes (FIG, SLC34A2, CD74) have been identified.  Resulting activation of ROS1 kinase activity appears to be a principal growth driver in these tumors. This kinase is sensitive to crizotinib, and patients with ROS1-fusion positive tumors have shown responses to this inhibitor. A break-apart FISH probe to the ROS1 locus can be used to screen for these fusions by FISH.

Recommended Use:
The ROS1 FISH test is recommended for screening NSCLC cases that are negative for EGFR mutation and ALK fusion.


FISH is performed on sections of formalin-fixed, paraffin-embedded tumor tissue.   Slides are prepared per standard protocols and 100 interphase cells are scored.

Specimen Requirements:

Formalin-fixed paraffin-embedded tumor block or FFPE slides:  

  • If sending slides, please include H&E stained slide cut from same block
  • Preferred slice thickness is 4-5 microns on positively charged slides. 
  • Please submit 3-5 slides.  Store at room temperature.
  • Contact Client Services at (855) 535-1522 for shipping supplies and instructions.

Test Performed (Days):


Turn Around Time:

5-7 days

Shipment Sensitivity Requirements:

  • Ship via overnight express, using the FedEx priority overnight label provided. 
  • Contact Client Services at (855) 535-1522 for shipping kits and instructions.


Additional Info: