Background:
Gene fusions involving ROS1 are present in 1-2% of non-small cell lung carcinomas, primarily adenocarcinomas. Several partner genes (FIG, SLC34A2, CD74) have been identified. Resulting activation of ROS1 kinase activity appears to be a principal growth driver in these tumors. This kinase is sensitive to crizotinib, and patients with ROS1-fusion positive tumors have shown responses to this inhibitor. A break-apart FISH probe to the ROS1 locus can be used to screen for these fusions by FISH.
Recommended Use:
The ROS1 FISH test is recommended for screening NSCLC cases that are negative for EGFR mutation and ALK fusion.
Methodology:
FISH is performed on sections of formalin-fixed, paraffin-embedded tumor tissue. Slides are prepared per standard protocols and 100 interphase cells are scored.
Specimen Requirements:
Formalin-fixed paraffin-embedded tumor block or FFPE slides:
- If sending slides, please include H&E stained slide cut from same block
- Preferred slice thickness is 4-5 microns on positively charged slides.
- Please submit 3-5 slides. Store at room temperature.
- Contact Client Services at (855) 535-1522 for shipping supplies and instructions.
Test Performed (Days):
Mon-Sat
Turn Around Time:
5-7 days
Shipment Sensitivity Requirements:
- Ship via overnight express, using the FedEx priority overnight label provided.
- Contact Client Services at (855) 535-1522 for shipping kits and instructions.
References:
Additional Info: