• Test Code:
    8692
  • Department:
  • Test Synonyms:
    Multiple MyelomaRetinoblastoma13q-del(13)(q14)
  • CPT Code(s):
    88271882758368 FFPE
Background:

Fluorescent in situ hybridization (FISH) with the RB1/D13S319 probe set detects deletion in 13q14.   These 13q14 deletions may be observed in multiple myeloma and leukemia.  Constitutional deletion of this region has been associated with retinoblastoma.

Methodology:

Slides are prepared per standard protocols. 10 metaphase cells are scored per probe.  This FISH test is used as a supplement to standard G-banded chromosome analysis.

Specimen Requirements:

  • Bone Marrow:  Shortly before aspiration add 0.2 cc of Sodium Heparin (1,000 units/ml) to tube of transport medium (please contact lab to arrange for transport medium to be sent).  Add at least 1 cc of bone marrow aspirate to the tube and suspend well.  Allow no clots to form.
  • Peripheral blood: May be used if bone marrow is inaspirable and blasts (.5%) are present or for constitutional deletion studies.  Send in Sodium Heparin tube.
  • Bone core biopsy: May also provide cells in cases where marrow is severely packed.  Send in transport medium with Sodium Heparin.
  • Lymph node or tumor: Tumor specimens of at least 0.5 cm3 (up to 3 inches in diameter) are immediately collected with sterile methods into closable containers with sterile transport medium.* Needle biopsies will be accepted, but are often difficult to grow.  Deliver the specimen in transport medium to the laboratory within a day, if possible, with decreased success rates as specimens are delayed in transit.  Protect sample from temperature extremes during shipping.

*Sterile Ringer’s solution, either lactated or non-lactated and sterile isotonic saline are alternatives, if no complete RPMI is available.  Contact Client Services for more information on media requirements.
It is preferable to collect the specimen before initiation of chemotherapy in the patient.  Tumor samples should be selected from viable areas, with as little normal or necrotic material as possible. 
Fluid samples (e.g., ascitic or pleural fluid) do not require culture medium for collection, but should be collected and transported aseptically. 
Unacceptable specimens are acellular, necrotic specimens, septic specimens, specimens in fixative or frozen, or specimens collected more than one week previously.

  • Formalin-fixed paraffin-embedded tumor block or FFPE slides
    • If sending slides, please include H&E stained slide cut from same block
    • Preferred slice thickness is 4-5 microns on  positively charged slides. 
    • Please submit 3-5 slides.  Store at room temperature.
    • Contact Client Services for more information.

A REQUISITION FORM MUST ACCOMPANY ALL SAMPLES. Please provide detailed clinical information.

Test Performed (Days):

Mon - Sat

Turn Around Time:

3 - 7 days

Shipment Sensitivity Requirements:

Ship via overnight express, using the FedEx priority overnight label provided.  Use cold pack to keep specimens cool, but not frozen.  Contact Client Services at (855) 535-1522 for shipping kits and instructions.

References:

Additional Info:

The Knight Cancer Institute at Oregon Health & Science University is a pioneer in the field of precision cancer medicine. The institute's director, Brian Druker, M.D., helped prove it was possible to shut down just the cells that enable cancer to grow. This breakthrough has made once-fatal forms of the disease manageable and transformed how cancer is treated. The OHSU Knight Cancer Institute is the only National Cancer Institute-designated Cancer Center between Sacramento and Seattle – an honor earned only by the nation's top cancer centers. It is headquarters for one of the National Cancer Institute's largest research collaboratives, SWOG, in addition to offering the latest treatments and technologies as well as hundreds of research studies and clinical trials.

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