• Test Code:
  • Department:
  • Test Synonyms:
    Maternal Cell Contamination Testing Maternal Cell Rule-Out MCC
  • CPT Code(s):

The presence of maternal cell contamination (MCC) in amniotic fluid or chorionic villus samples poses a serious preanalytical risk for prenatal misdiagnosis.  This test is used to rule out the presence of maternal cell contamination within a fetal specimen1


Polymerase chain reaction:  (PCR)-based comparison of multiple microsatellite markers in maternal vs. fetal samples.

Specimen Requirements:

Both maternal and fetal specimens required:

  • Blood - Maternal:
    • 6 mL - EDTA (purple-top) or ACD (yellow-top) tube
    • If sending DNA: 100ng at a minimum of 100ng/µL
  • Amniotic Fluid - Fetal:
    • 10 mL direct amniotic fluid, OR
    • 2 T-25 flasks of cultured amniocytes (fully confluent)
    • If sending DNA: 100ng at a minimum of 100ng/µL


  • Chorionic Villus - Fetal:
    • 10 -20 mg direct chorionic villus (CVS), OR
    • 2 T-25 flasks of cultured CVS cells (fully confluent)

    • If sending DNA: 100ng at a minimum of 100ng/µL

A REQUISITION FORM MUST ACCOMPANY ALL SAMPLES.  Please include detailed clinical information, including ethnicity, clinical history, and family history.

Test Performed (Days):


Turn Around Time:

10-14 Days

Shipment Sensitivity Requirements:

  • Package and ship specimen to remain cold, but not frozen. 
  • Ship via overnight express, using the FedEx priority overnight label provided. 
  • Contact Client Services for shipping kits and instructions at (855) 535-1522.


1. Schrijver, I. et al. Testing for Maternal Cell Contamination in Prenatal Samples.  J Mol Diagn. 2007 July; 9(3): 394–400.

Additional Info:

The lower level of sensitivity of the assay is approximately 5% maternal material in the fetal specimen.  

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