Background:
This FISH panel is designed to detect the most common, and/or prognostically-significant abnormalities in HES, CMML and MPN (Swerdlow et al. 2008). FISH studies are useful adjuncts to complete chromosome studies, particularly when following an abnormal clone, assessing relapse and progression, or when material is inadequate for chromosomal analysis.
The HES/CMML/MPN FISH panel includes:
- FIP1LI-CHIC2-PDGFRA, to detect FIP1L1-PDGFRA fusion due to CHIC2 deletion.
- FGFR1 break-apart probe, to detect FGFR1 (8p12) rearrangement.
- PDGFRB break-apart probe, to detect PDGFRB (5q33.1) rearrangement.
- BCR/ABL + ASS dual-fusion probe, to detect t(9;22) and variants.
- JAK2 break-apart probe, to detect JAK2 (9p24) rearrangement.
- ETV6 break-apart probe, to detect ETV6 (TEL) (12p13) rearrangement.
- D7S486/CEP 7 probe set, to detect monosomy 7 and 7q deletion.
- CEP 8, to detect trisomy 8.
Note: Interphase FISH analysis is not intended to stand alone, but rather to provide supplemental information to routine cytogenetic studies.
Methodology:
Slides are prepared per standard protocols and 100-200 interphase cells are scored per probe.
*Please note: it is our laboratory policy to analyze at least one metaphase cell from every hybridization, if possible, including both normal and abnormal cells, if both exist.
Specimen Requirements:
The HES/CMML/MPD FISH Panel can be performed on the following specimen types. After collection, keep at room temperature until shipping. Contact Client Services at (855) 535-1522 for shipping kits and instructions.
- Bone Marrow: Shortly before aspiration add 0.2 cc of Sodium Heparin (1,000 units/ml) to tube of transport medium (please contact lab to arrange for transport medium to be sent). Add at least 1 cc of bone marrow aspirate to the tube and suspend well. Allow no clots to form.
- Peripheral blood: May be used if bone marrow is inaspirable and blasts (.5%) are present. Send in Sodium Heparin tube.
- Bone core biopsy: May also provide cells in cases where marrow is severely packed. Send in transport medium with Sodium Heparin.
- Peripheral blood or Bone marrow smear: Provide 10-16 smears.
A REQUISITION FORM MUST ACCOMPANY ALL SAMPLES. Please include detailed clinical information.
Test Performed (Days):
Mon - Sat
Turn Around Time:
5 – 10 days Contact Lab at 855-KDL-1LAB (535-1522) - Time varies depending on number of tests performed.
Shipment Sensitivity Requirements:
- Keep specimen at room temperature during transit.
- Do not use the cold pack provided in the KDL shipping kit.
- Ship the specimen overnight express, using the FedEx priority overnight label provided.
- The specimen must arrive at the lab no more than 24 hours after collection.
References:
- Swerdlow et al. (Eds.): WHO Classification of Tumors of Haematopoietic and Lymphoid Tissues. IARC: Lyon 2008
Additional Info: