Background:
Knight Diagnostic Laboratories have an extensive menu of probes for hematologic and solid tumor analyses by fluorescent-in situ-hybridization (FISH) techniques. Karyotypic analysis and FISH testing are key components in the diagnosis of hematologic malignancies. Recently, FISH has become an essential component in therapeutic decisions for solid tumor patients as well. Tests are developed quickly after clinical utility has been established.
Methodology:
Slides are prepared per standard protocols and 100-200 interphase cells are scored for each probe under fluorescence microscopy. FISH can also be performed on sections of formalin-fixed, paraffin-embedded tumor tissue; slides are prepared per standard protocols and 100 interphase cells are scored. For tumor samples on FFPE, testing includes a pathologist review of each tumor slide prior to FISH testing. For multiple myeloma, blood or bone marrow cells are sorted on CD138+ prior to FISH.
Specimen Requirements:
Bone Marrow:
Shortly before aspiration add 0.2 cc of Sodium Heparin (1,000 units/ml) to tube of transport medium (please contact lab to arrange for transport medium to be sent). Add at least 1 cc of bone marrow aspirate to the tube and suspend well. Allow no clots to form.
Peripheral blood:
May be used if bone marrow is inaspirable and blasts (.5%) are present. Send in Sodium Heparin tube.
Bone core biopsy:
May also provide cells in cases where marrow is severely packed. Send in transport medium with Sodium Heparin.
Bone marrow smears:
Provide 10 slides
Formalin-fixed paraffin-embedded tumor block or FFPE slides:
- If sending slides, please include H&E stained slide cut from same block
- Preferred slice thickness is 4-5 microns on positively charged slides.
- The number of slides needed will change based on the number of probes used. Please submit 3-5 slides for a single probe test, with an additional 1-2 slides for each additional probe to be tested.
- Store at room temperature.
- Contact Client Services at (855) 535-1522 for shipping supplies and instructions
A REQUISITION FORM MUST ACCOMPANY ALL SAMPLES. Please include detailed clinical information, including ethnicity, clinical history, and family history.
Test Performed (Days):
Mon - Sat
Turn Around Time:
3-7 days* *TAT may be extended if part of a chromosome study
Shipment Sensitivity Requirements:
- Ship via overnight express, using the FedEx priority overnight label provided.
- Contact Client Services at (855) 535-1522 for shipping kits and instructions.
References:
Additional Info: