Solid Tumors
RNA Transcriptome
Background:
This test is designed to sequence mRNA from solid tumors for transcriptome analyses. The resulting data are used to screen for fusion genes and to assess the expression of gene variants/mutations (for correlation with tumor DNA sequencing results). The data may also be used for pathway analyses and other analytics, but such analyses are not included in the clinical report.
Reasons for Referral: Advanced stage solid tumor.
Methodology:
The tumor is examined microscopically and tumor-rich areas are identified for dissection and RNA extraction. Ideally, the submitted material should contain 50% or more tumor fraction. After purification and quantitation, the RNA is converted to cDNA and sequenced using an Illumina RNA Exome kit run on an Illumina next generation sequencing platform.
Specimen Requirements:
- A paraffin block, or
- 10 unstained sections of tumor (4-5 microns)(15 sections for small biopsies).Contact
- Client Services for shipping materials and procedures at (855) 535-1522.
Test Performed (Days):
Weekly
Turn Around Time:
21-28 days
Shipment Sensitivity Requirements:
- Keep specimen cool during transit, but do not ship on dry ice.
- Please use the cold pack provided in the KDL shipping kit.
- Ship the specimen overnight express, using the FedEx priority overnight label provided.
- Contact Client Services for shipping materials and procedures at (855) 535-1522.
References:
Additional Info: