Solid Tumors Quantitative Intracellular Signaling Panel - Research only

***Only Availalbe as a Research Test***

This test is based on Nanostring’s Vantage 3D Protein Solid Tumor Panel (FFPE), which consists of a cocktail of 25 oligonucleotide-tagged antibodies designed to bind to key proteins involved in intracellular signaling or to specific sites of phosphorylation on these proteins (see table below). The panel includes non-specific mouse IgG1 and rabbit IgG antibodies as controls for background binding (total of 27 antibodies). Samples of FFPE cancer cell lines are included in each run, which serve as controls for antibody performance and for inter-run data normalization.

The panel is validated only for core biopsies of breast cancer or prostate cancer. Other samples types, including excisional biopsies and resections, are not acceptable for this assay due to the known lability of protein phosphorylation. Results are reported relative to a cohort of previously analyzed solid tumors that match the pathologic diagnosis of the submitted sample.

4E-BP1	Phospho-MEK1/2
EGF Receptor	Phospho-PDK1
GSK-3B	Phospho-PRAS40
HER2	Phospho-S6 Ribosomal Protein
Ki-67	Phospho-c-Raf
Met	Phospho-p44/42 MAPK ERK 1/2
Pan-Akt	Progesterone Receptor
Pan-Keratin	S6 Ribosomal Protein
Phospho-4E-BP1	p44/42 MAPK ERK1/2
Phospho-AMPKa	P53
Phospho-Akt	Histone H3
Phospho-EGF Receptor	MmAb-IgG1
Phospho-GSK-3B	RbAb-IgG
Phospho-Histone H3

1. Microscopic examination of the specimen to confirm presence of adequate tumor
2. Overnight binding of oligonucleotide-tagged antibody cocktail
3. UV-release of oligonucleotides
4. Detection/counting of oligonucleotides using Nanostring MAX nCounter

• Only core biopsy specimens are acceptable.
• 1 paraffin block or 2 unstained sections of tumor (4-5 microns).
• If shipping materials are needed, contact Client Services at (855) 535-1522.

A REQUISITION FORM MUST ACCOMPANY ALL SAMPLES.  Please include detailed clinical information.

Mon-Fri

7-10 days