Cancer FISH
NSCLC FISH Panel (ALK, RET, ROS1, MET)
Background:
Screening for mutations in oncogenes and tumor suppressor genes is increasingly important in delivering personalized cancer care. The GeneTrails® NSCLC Panel delivers sequencing information on a variety of treatment-informative mutations in 23 genes, which are known to play a role in cases of non-small cell lung cancer.
In addition to the sequencing panel, tumor samples can be screened by FISH for translocations involving the ALK, ROS1 or RET kinase genes, and for MET gene amplification. This FISH panel may be added to the sequencing panel by ordering both test codes 5120 and 7600. The FISH probes may be ordered individually as well: ALK (test code 8019), ROS1 (test code 8720), RET (test code 8700) and MET (test code 8500).
Methodology:
The GeneTrails® NSCLC FISH Panel is performed on DNA from formalin-fixed, paraffin-embedded (FFPE) tumor tissue. FISH slides are prepared per standard protocols and 100 interphase cells are scored (50 cells for MET).
Specimen Requirements:
- A paraffin block or
- 4-8 unstained sections of tumor (4-5 microns) on positively charged slides
A REQUISITION FORM MUST ACCOMPANY ALL SAMPLES. Please include detailed clinical information.
Test Performed (Days):
Mon - Sat
Turn Around Time:
FISH: 5 - 7 days
Shipment Sensitivity Requirements:
- Package and ship specimen to remain cool, but not frozen.
- Ship via overnight express, using the FedEx priority overnight label provided.
- Contact Client Services at (855) 535-1522 for shipping kits and instructions.
References:
Additional Info:
Recommended Use:
The GeneTrails® NSCLC FISH Panel is well-suited for screening potentially actionable mutations in the non-small cell lung carcinomas, including adenocarcinomas, squamous cell carcinomas, and large cell undifferentiated carcinomas.