Cancer Cytogenetics
Heme/Onc Blood: Full Chromosome Study reflexed to FISH
Background:
Numerical and structural chromosome changes are a recognized phenomenon in cancer progression. While bone marrow is the specimen of choice, unstimulated peripheral blood is an acceptable alternative when bone marrow aspiration is not possible. Blood chromosome analysis, as a companion to pathological assessment, may aid in the diagnosis and classification of malignant hematological disease. The specific chromosome aberration may be associated with prognosis and may be useful in directing therapy. Continued monitoring of bone marrow chromosomes through treatment and remission allows assessment of disease status and the opportunity to observe clonal progression. Companion fluorescent in situ hybridization (FISH) studies with appropriate probe sets are valuable in further delineation of chromosome abnormalities and in assessing minimal residual disease.
Methodology:
- Chromosome analysis (karyotyping) with banding (<400-450 bands)
- Reflex testing to FISH as appropriate to confirm diagnosis.
Specimen Requirements:
Blood:
- Adult - 3-5 mL drawn into a GREEN top sodium heparin vacutainer tube or into a pre-heparinized plastic syringe (use 0.2 cc sodium heparin, 1000 unit/mL). Do NOT use lithium heparin.
- Child - 1-2 mL, as above.
- Infant - 1-2 mL, as above.
- Keep at room temperature and transport to laboratory as soon as possible.
- Contact Client Services at (855) 535-1522 for supplies and instructions.
A REQUISITION FORM MUST ACCOMPANY ALL SAMPLES. Please include detailed clinical information, including ethnicity, clinical history, and family history.
Test Performed (Days):
Mon - Sat
Turn Around Time:
7 – 10 Days
Shipment Sensitivity Requirements:
- Please contact Client Services at (855) 535-1522 for shipping kits and instructions.
- Do not use the cold pack provided in the KDL shipping kit.
- Ship the specimen overnight express, using the FedEx priority overnight label provided.
- The specimen must arrive at the lab no more than 24 hours after collection.
References:
Additional Info:
After hours, please leave message at (855) 535-1522 for cytogeneticist on call.