Solid Tumors
GeneTrails Solid Tumor Fusion Gene Panel
Background:
In recent years an increasing number of gene fusions have been identified in solid tumors, many of them involving kinases that are targetable with specific therapies. Examples include fusions involving ALK (e.g. EML4-ALK) and ROS1 in non-small cell carcinoma, RET in thyroid carcinoma, BRAF in some types of glioma, and FGFR2 in cholangiocarcinoma.1-6
The GeneTrails® Solid Tumor Fusion Gene panel is designed to detect fusions involving the 21 target genes listed below. The panel is performed using next-generation sequencing of tumor RNA, and is useful for finding clinically actionable fusions that are not routinely screened by FISH. Control genes are included on the panel to assess specimen adequacy. The assay has high sensitivity (to the level of approximately 1%, or 1 positive cell in 100).
|
Fusion genes
|
|
AKT3
|
ALK
|
BRAF
|
EGFR
|
ERBB4
|
ERG
|
FGFR1
|
|
FGFR2
|
FGFR3
|
MET
|
NOTCH1
|
NOTCH2
|
NRG1
|
NTRK1
|
|
NTRK2
|
NTRK3
|
NUTM1
|
PDGFRA
|
RAF1
|
RET
|
ROS1
|
Methodology:
- Microscopic examination of the specimen and macrodissection of tumor-rich areas
- Nucleic extraction and purification
- Next-generation RNA sequencing
Specimen Requirements:
- A paraffin block or
- 10 unstained sections of tumor (4-5 microns)(15 sections for small biopsies).
- Contact Client Services for shipping materials and procedures at (855) 535-1522.
A REQUISITION FORM MUST ACCOMPANY ALL SAMPLES. Please include detailed clinical information.
Test Performed (Days):
Mon - Fri
Turn Around Time:
10-17 days
Shipment Sensitivity Requirements:
- Keep specimen cool during transit, but do not ship on dry ice.
- Please use the cold pack provided in the KDL shipping kit.
- Ship the specimen overnight express, using the FedEx priority overnight label provided.
- Contact Client Services for shipping materials and procedures at (855) 535-1522.
References:
- Shaw AT, et al. Ceritinib in ALK-rearranged non-small-cell lung cancer. N Engl J Med. 2014 Mar 27;370(13):1189-97.
- Shaw AT, et al. Crizotinib versus chemotherapy in advanced ALK-positive lung cancer. N Engl J Med. 2013 Jun 20;368(25):2385-94.
- Shaw AT, et al. Crizotinib in ROS1-rearranged non-small-cell lung cancer. N Engl J Med. 2014 Nov 20;371(21):1963-71.
- Elisei R, et al. Cabozantinib in progressive medullary thyroid cancer J Clin Oncol. 2013 Oct 10;31(29):3639-46.
- Dimitriadis E, et a. BRAF alterations in pediatric low grade gliomas and mixed neuronal-glial tumor. J Neurooncol. 2013 Jul;113(3):353-8.
- Graham RP, et al. Fibroblast growth factor receptor 2 translocations in intrahepatic cholangiocarcinoma Hum Pathol. 2014 Aug;45(8):1630-8
Additional Info: