Cancer FISH • Constitutional FISH 8 Centromere FISH

The chromosome 8 centromere probe is used in conjunction with standard cytogenetic analysis for identifying and enumerating chromosome 8 via fluorescence in situ hybridization (FISH) in interphase nuclei and in metaphase spreads.  The assay is appropriate for cells obtained from bone marrow and peripheral blood of patients with myeloid disorders :  (Chronic  Myelogenous Leukemia (CML), Acute Myeloid Leukemia (AML), Myeloproliferative Disorder (MPD), Myelodysplastic Syndrome (MDS), and other hematological disorders.  The centromere probe may be used in a single assay or as part of a FISH panel.  It is also used to detect constitutional trisomy 8 mosaicism syndrome.

Slides are prepared per standard protocols and 200 interphase cells are scored.
*Please note: it is our laboratory policy to analyze at least one metaphase cell in an interphase FISH study, if possible, including both normal and abnormal cells if both exist.

Blood:  

• Adult - 3-5 mL drawn into a GREEN top sodium heparin vacutainer tube or into a pre-heparinized plastic syringe (use 0.2 cc sodium heparin, 1000 unit/mL).  Do NOT use lithium heparin.
• Child - 1-2 mL, as above.
• Infant - 1-2 mL, as above. 
• Keep at room temperature and transport to laboratory as soon as possible.
• Contact Client Services at (855) 535-1522 for supplies and instructions.
  • Bone Marrow:  Shortly before aspiration add 0.2 cc of Sodium Heparin (1,000 units/ml) to tube of transport medium (please contact lab to arrange for transport medium to be sent).  Add at least 1 cc of bone marrow aspirate to the tube and suspend well.  Allow no clots to form.
  • Peripheral blood: May be used if bone marrow is inaspirable and blasts (.5%) are present, or for constitutional blood studies.  Send in Sodium Heparin tube.
  • Bone core biopsy: May also provide cells in cases where marrow is severely packed.  Send in transport medium with Sodium Heparin.
  • Bone marrow smears:  Provide 10 slides

A REQUISITION FORM MUST ACCOMPANY ALL SAMPLES. Please provide detailed clinical information.

Mon - Sat

3-7 days

Ship via overnight express, using the FedEx priority overnight label provided.  Contact Client Services at (855) 535-1522 for shipping kits and instructions.